Coding

Part:BBa_K3237008:Design

Designed by: Aubrey Demchuk, Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-14)


His-mRFP1-proinsulin-CPP with furin cut sites codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 363
    Illegal XhoI site found at 904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon optimized for C.reinhardtii . our project concerns itself with expressing insulin in micro algae. therefore, this construct was made for comparative purposes.

Source

This part is based on Tsinghua2014 and the mRFP1 from the iGEM registry BBa_E1010. The part was synthesized by TWIST.

References

1. Liang, J., and Yang, V. (2005) Insulin-cell penetrating peptide hybrids with improved intestinal absorption efficiency. Communications. 335, 734-738.

2. Hay, C., and Docherty, K. (2003) Enhanced expression of a furin-cleavable proinsulin. Journal of molecular endocrinology. 3, 597-607.